\log
The initial iteration of this file was generated, on 2 June 1995 by Rick
Ingersoll, from a 21 April 1995 email message from David Lipson, and the
file was put under sccs control.[RCI 2 June 1995] Additional details
regarding methodology were provided by David Lipson and these were
incorporated into the documentation section on 5 June 1995.[RCI 5 June
1995] URL locations for related data were updated on 27 September
2000.[MAH 27 September 2000] The header metadata for cols 4, 5, 6, 7,
14, and 17 was updated to reflect more consistent unit definitions.[TMA
09 August 2004]
\doc
TITLE. Luxury uptake and storage of nitrogen in the rhizomatous alpine
herb, Bistorta bistortoides.
ABSTRACT. The patterns of luxury consumption and storage of nitrogen were
studied in the rhizomatous alpine herb, Bistorta bistortoides (Pursh.).
Emphasis was placed on understanding the mechanisms, carbon costs, and
possible benefits to growth of assimilating and storing excess nitrogen.
Nitrogen pools in the rhizome and shoot were measured in fertilized and
non-fertilized plants over two growing seasons (the 3rd and 4th growing
seasons after initiation of fertilization). Fertilized plants stored more
N in the rhizome throughout both seasons and exhibited greater mobilization
of N reserves to support growth, compared to non-fertilized plants. Flower,
foliar, and preformed bud N concentrations and biomass production rate did
not increase in response to fertilization. The increased storage of N
without a concomitant influence on biomass production demonstrates that
luxury consumption occurs in this species. Fertilized plants relied more
heavily on stored N to drive seasonal growth, with translocation from
reserves accounting for 56% and 100% of the aboveground N requirement in
non-fertilized and fertilized plants, respectively. In fertilized plants,
the complete reliance on stored N indicates that the increase in
utilization of N from reserves is accompanied by a decrease in utilization
of N from current-season uptake. Total plant N content in fertilized
plants did not change throughout the entire growing season, demonstrating
the absence of net N uptake from the soil. Thus, the uptake and storage of
excess N was accompanied by negative feedback to further soil N uptake.
Such feedback would constrain the continued accumulation of excess N and
eliminate any long term benefits of luxury N uptake in this species.
Increased N storage in fertilized plants was accommodated by an increase
in the amino acids arginine, the non-protein amino acid,
delta-acetyl-ornithine, glutamine, and glutamate. It was found that the
entire length of the rhizome, including tissues that are several decades
old, and both the pith and the cortex are active in N storage. Luxury
uptake was accommodated entirely through filling of existing rhizome cells;
no increased cell proliferation was found. Sucrose concentration was
significantly lower in the recently produced rhizome tissue of fertilized
plants, reflecting the increased, direct carbon costs of storing the
additional N.
There do not appear to be growth benefits associated with luxury N uptake
in this species. In response to luxury uptake a switch occurs, such that
growth is driven entirely by stored, excess N, rather than a combination
of stored N and N assimilated during the current season. By relying
entirely on stored N, the cost of root construction and nutrient
assimilation should be reduced. However, this apparent benefit is offset
by (1) inherent developmental constraints to the rate of growth in this
species which prevents the utilization of excess N to drive increased
growth, (2) negative feedback between luxury uptake and future N
assimilation, and (3) substantial direct carbon costs of storing the
excess N. These costs and constraints appear to balance any benefits to
luxury uptake, resulting in no net response of plant growth.
Delta-acetyl-ornithine was found to vary seasonally and in response to
nitrogen availability in the rhizomes of the alpine herb, Bistorta
bistortoides (Pursh). Delta-acetyl-ornithine accounts for 38% of the free
amino acid pool and up to 12% of the total nitrogen in rhizomes. It is
concluded that this compound plays an important role in nitrogen storage
in this species.
INVESTIGATOR. David Lipson, EPOB, CB 334, University of Colorado,
Boulder, CO, 80309-0334, (303) 492-6319, lipson@culter.colorado.edu.
VARIABLES. rhizome dry weight, rhizome nitrogen content, rhizome amino
acid content, inflorescence dry weight, inflorescence nitrogen content,
shoot dry weight, shoot nitrogen content.
KEYWORDS. luxury consumption, Bistorta bistortoides, biogeochemistry, nutrient cycling, nitrogen, allocation, amino acids, delta-acetyl-ornithine, Biogeochemistry, vegetation pools, disturbance.
LOCATION. Study area consisted of fertilized and control plots in the
Niwot Ridge Saddle. Original data are stored with David Lipson.
TIMING. begin 28 July 1994, end 3 September 1994, approximately once
per month during the field season.
CITATIONS. [1]Rosen, H. 1957. A modified ninhydrin colorimetric analysis
for amino acids. Archives of Biochemistry and Biophysics 67: 10-15.
[2]Jones, B.N., S. Paabo, and S. Stein. 1981. Amino acid analysis and
enzymatic sequence determination of peptides by an improved
o-phtaldialdehyde precolumn labeling procedure. Journal of Liquid
Chromatography 4: 565-586.
[3]May, D.E., and P.J. Webber. 1982. Spatial and temporal
variation in the vegetation and its productivity on Niwot Ridge,
Colorado. In: Halfpenny, J.C., (ed.), Ecological Studies in the Colorado
Alpine. Institute of Arctic and Alpine Research Occasional Paper 37:
35-62.
[4]Carpenter, J.F., and S.C. Hand. 1986. Arrestment of carbohydrate
metabolism during anaerobic dormancy and aerobic acidosis in Artemia
embryos: Determination of pH-sensitive control points. Journal of
Comparative Physiology B 156: 451-459.
[5]Jaeger, C.H., and R.K. Monson. 1992. Adaptive significance of nitrogen
storage in Bistorta bistortoides, an alpine herb. Oecologia 92: 578-585.
[6]Bowman, W.D., T.A. Theodose, and M.C. Fisk. 1995. Physiological and
production responses of plant growth forms to increases in limiting
resources in alpine tundra: implications for differential community
responses to environmental change. Oecologia 101: 217-227.
[7]Lipson, D.A., W.D. Bowman, and R.K. Monson. In press.
Luxury uptake and storage of nitrogen in the rhizomatous alpine herb,
Bistorta bistortoides. Ecology.
[8]Lipson, D.A., T.K. Raab, and R.K. Monson. 1996. Delta-acetylornithine
as a major nitrogen storage compound in Bistorta bistortoides. Phytochemistry
41 (1):29-30.
COMMENTS. The source material for the initial iteration of this file
was a 21 April 1995 email message from David Lipson to Rick Ingersoll.
The an ASCII version of this email message has been archived as
/data1/Datman/Lipson/Archive/luxupt on culter, the NWT LTER workstation.
Some of the methodology documentation was provided in a second, 5 June
1995, email message which has been archived as
/data1/Datman/Lipson/Archive/mm on culter.
The site for this study was within a moist meadow community (as defined
by May and Webber, 1982) dominated by the forb Acomastylis rossii and the
graminoid Deschampsia caespitosa, and sub-dominated by B. bistortoides.
The meadow lies on the western side of the saddle (the "Saddle") formed
by two knolls on Niwot Ridge in the Front Range of the Colorado Rocky
Mountains.
Observations were made on plants in N fertilized and control (non-
fertilized) plots. The fertilization treatment was applied over the
two-year period of 1991-1992 as described previously (Bowman et al. 1995).
Five replicate 1 m2 plots were fertilized with biweekly additions of 2 g
NH4NO3 dissolved in 5 L of deionized water. The meadow surrounding the
plots served as a non-fertilized, unwatered control. Bowman et al. (1995)
found no effect of water alone on biomass or leaf gas exchange in Bistorta
bistortoides in these plots. The current study was conducted during 1993
and 1994, 1-2 years after fertilization had ceased.
In 1993, the first year after cessation of fertilization, plants from
fertilized and non-fertilized plots were collected at the time of full
bloom at mid-season (27 July) and at the time of leaf senescence at the
end of the season (16 September). In 1994, fertilized and control plants
were collected just after snowmelt when leaves were still unexpanded
(25 May-1 June), at mid-season (6 July), when plants were in full bloom,
and at the end of the season (3 September). Plants were also collected on
18 August, when leaves were partially senesced, for the comparison of N
concentrations in cortex and pith of rhizomes. Non-fertilized control
plants were collected on two additional dates (22 June, 26 July) during
the season. On each sampling date, 4-8 plants from both treatments were
completely excavated from the soil. The rhizome and fine roots of B.
bistortoides are rather shallow, making it easy to harvest the entire
plant.
Within 6 hours of collection, plants were washed in distilled water, fine
roots and previous years' dead tissue were removed, aboveground portions
were separated from the rhizome and divided into leaves and the
inflorescence (including the stalk and attached leaves) for mid-season
plants, or were divided into current-year senescent tissue and the
preformed bud for end-of-season plants. All aboveground tissues were dried
at 70 degrees Celsius for 48 h.
The rhizome was weighed and split into three transverse sections of similar
fresh weight (proximal, medial, and distal, or alternatively, from youngest
to oldest). Each section was then split in half lengthwise. One half of
each section was weighed and dried at 70 degrees Celsius for water content
and total Kjeldahl N determination, while the remaining half of each
section was stored at -20 degrees Celsius and used for later analysis.
Frozen tissues were ground in liquid N with a mortar and pestle. Samples
(100-200 mg) were extracted with 1 ml 6% perchloric acid as described by
Carpenter and Hand (1986). The insoluble pellet was dried at 70 degrees
Celsius. Total amino acid concentration in the extracts was quantified
using a ninhydrin method (Rosen 1957), measuring absorbance at 562 nm on an
automatic plate reader (Molecular Devices Corporation). Analysis of
individual amino acids was performed by high-pressure liquid
chromatography (HPLC) on a C-18 column after derivitization of the samples
with o-Phthalodialdehyde (Jones et al. 1982). Total Kjeldahl N
concentrations of all tissues and fractions were determined as described by
Jaeger and Monson (1992).
Identification of the non-protein amino acid was performed by H-NMR
(solvent=D2O; frequency=300 MHZ; internal standard=TMS) and verified by
acid hydrolysis of the amino acid and subsequent analysis by HPLC (as
described above). The compound was extracted from frozen rhizomes ground in
liquid N2 in 80% MeOH, and isolated using paper chromatography with a
solvent system of phenol:H2O (4:1 w/w). Crystals were rinsed with diethyl
ether. Acid hydrolysis was performed in 6 M HCl, at 100 degrees Celsisu for
24 hr.
Averages for the entire rhizome (as determined from the proximal, medial,
and distal sections) are reported as "rhizome".
Fertilization treatment codes are 0 (control) and 1 (fertilized).
For date 090394, the "inflorescence" data are actually for the preformed
bud. On date 091693, the preformed bud was included with the "shoot" data.
The 5 samples from the control plots for which delta-acetyl-ornithine
concentrations are reported, and actually collected on 060194, are
here reported as being collected on 052594 in order to allow comparison
between fertilized and control specimens.
The absence of some data for a given sample can indicate that the
corresponding laboratory analyses were not performed, in addition to
being representative of "missing" data.
Nitrogen storage data for several alpine plants (including B. bistortoides)
on Niwot Ridge can be found in
http://culter.colorado.edu/exec/.extracttoolA?nitrstor.jj
\type
statistical.
\header
COL1. label=date (mmddyy), type=integer, units=none, missing value indicator=,
minimum=072893, maximum=090394, precision=1
COL2. label=fertilization treatment code, type=integer, units=none, missing
value indicator=, minimum=0, maximum=1, precision=1
COL3. label=rhizome dry weight, type=real, units=grams, missing value
indicator=absence of value, minimum=, maximum=, precision=
COL4. label=proximal rhizome total Kjeldahl nitrogen concentration (mg N/g dry tissue),
type=real, units=mg/g, missing value indicator=absence of value,
minimum=, maximum=, precision=
COL5. label=medial rhizome total Kjeldahl nitrogen concentration (mg N/g dry tissue),
type=real, units=mg/g, missing value indicator=absence of value, minimum=,
maximum=, precision=
COL6. label=distal rhizome total Kjeldahl nitrogen concentration (mg N/g dry tissue),
type=real, units=mg/g, missing value indicator=absence of value, minimum=,
maximum=, precision=
COL7. label=mean rhizome total Kjeldahl nitrogen concentration (mg N/g dry tissue),
type=real, units=mg/g, missing value indicator=absence of value, minimum=,
maximum=, precision=
COL8. label=rhizome total nitrogen content, type=real, units=mg, missing
value indicator=absence of value, minimum=, maximum=, precision=
COL9. label=proximal rhizome amino acid concentration (micromoles/g dry tissue),
type=real, units=micromoles/g, missing value indicator=absence of value,
minimum=, maximum=, precision=
COL10. label=medial rhizome amino acid concentration (micromoles/g dry tissue),
type=real, units=micromoles/g, missing value indicator=absence of value,
minimum=, maximum=, precision=
COL11. label=distal rhizome amino acid concentration (micromoles/g dry tissue),
type=real, units=micromoles/g, missing value indicator=absence of value,
minimum=, maximum=, precision=
COL12. label=mean rhizome amino acid concentration, type=real,
units=micromoles/g dry tissue, missing value indicator=absence of value,
minimum=, maximum=, precision=
COL13. label=inflorescence dry weight, type=real, units=grams, missing
value indicator=absence of value, minimum=, maximum=, precision=
COL14. label=inflorescence total Kjeldahl nitrogen concentration (mg N/g dry tissue),
type=real, units=mg/g, missing value indicator=absence of
value, minimum=, maximum=, precision=
COL15. label=inflorescence total nitrogen content, type=real, units=mg,
missing value indicator=absence of value, minimum=, maximum=, precision=
COL16. label=shoot dry weight, type=real, units=grams, missing value
indicator==absence of value, minimum=, maximum=, precision=
COL17. label=shoot total Kjeldahl nitrogen concentration (mg N/g dry tissue),
type=real, units=mg/g, missing value indicator=absence of value,
minimum=, maximum=, precision=
COL18. label=shoot total nitrogen content, type=real, units=mg,
missing value indicator=absence of value, minimum=, maximum=, precision=
COL19. label=rhizome arginine concentration (micromoles/g dry tissue),
type=real, units=micromoles/g, missing value indicator=absence of value,
minimum=, maximum=, precision=
COL20. label=rhizome delta-acetyl-ornithine concentration (micromoles/g
dry tissue), type=real, units=micromoles/g, missing value indicator=absence
of value, minimum=, maximum=, precision=
COL21. label=rhizome glutamine concentration (micromoles/g dry tissue),
type=real, units=micromoles/g, missing value indicator=absence of value,
minimum=, maximum=, precision=
COL22. label=rhizome glutamate concentration (micromoles/g dry tissue),
type=real, units=micromoles/g, missing value indicator=absence
of value, minimum=, maximum=, precision=
COL23. label=rhizome aspartate concentration (micromoles/g dry tissue),
type=real, units=micromoles/g, missing value indicator=absence of value,
minimum=, maximum=, precision=
COL24. label=rhizome tyrosine concentration (micromoles/g dry tissue),
type=real, units=micromoles/g, missing value indicator=absence of value,
minimum=, maximum=, precision=
COL25. label=rhizome serine concentration (micromoles/g dry tissue),
type=real, units=micromoles/g, missing value indicator=absence of value,
minimum=, maximum=, precision=
COL26. label=rhizome asparagine concentration (micromoles/g dry tissue),
type=real, units=micromoles/g, missing value indicator=absence of value,
minimum=, maximum=, precision=
{*********************************************************************
If the data section below contains no data then you will need to
obtain permission from the investigator listed in the documentation
section of this file. If you have obtained permission or if the data
are already contained in this file, include the following
acknowledgment (in addition to an acknowledgment of the investigator)
in any published use of these data: "Logistical support
and/or data were provided by the Niwot Ridge Long-Term Ecological
Research project (NSF DEB 0423662) and the Mountain Research Station
(BIR 9115097)." Please send 4 reprints or high-quality copies of any
such publications to Todd Ackerman, NWT LTER data manager,
INSTAAR, CB 450, University of Colorado, Boulder, CO, 80309-0450.
The data contained in these files have many sources. Generally,
data collected prior to 1993 would have been subjected to quality
control standards imposed by the investigator. Data after 1992
were likely to have been manually entered and/or processed in the
NWT LTER central data management laboratory. Detailed information
on the quality control protocols and programs associated with any
data routed through the NWT LTER data management laboratory will
be supplied upon request. The user of these data should be aware
that, while efforts have been taken to ensure that these data are
of the highest quality, there is no guarantee of perfection for
the data contained herein and the possibility of errors exists.
If you encounter questionable data, please contact the NWT LTER
data manager (todda@culter.colorado.edu; (303) 492-4771) so that
the data can be corrected or qualified. Thus, these data should
be considered dynamic in that past data may be modified and future
data will be appended.
******************************************************************}
\data
072893,0,0.8694,5.93,4.78,5.46,5.25,4.56,,,,30.00,0.074,24.06,1.78,0.2989,28.15,8.41,,,,,,,,
072893,0,2.9039,11.69,6.01,6.34,8.00,23.23,,,,32.00,0.3693,24.8,9.16,0.6636,36.36,24.13,,,,,,,,
072893,0,1.2320,5.18,5.4,5.21,5.25,6.47,,,,14.00,0.1291,28.23,3.64,0.3433,37.15,12.75,,,,,,,,
072893,0,1.1574,7,5.44,6.8,6.27,7.26,,,,17.00,0.2567,35.53,9.12,0.2999,33.85,10.15,,,,,,,,
072893,0,1.9421,5.26,4.9,6.37,5.47,10.62,,,,16.00,0.1895,25.08,4.75,0.3849,36.27,13.96,,,,,,,,
072893,0,0.6758,8.15,7.12,5.96,7.03,4.75,,,,90.00,0.0763,22.13,1.69,0.3003,32.89,9.88,,,,,,,,
072893,0,0.8318,13.32,13.12,18.54,14.89,12.39,,,,112.00,0.0767,22.93,1.76,0.1812,37.66,6.82,,,,,,,,
072893,1,1.0164,19.76,14.17,10.04,15.89,16.15,,,,168.00,0.124,20.19,2.50,0.2767,36.67,10.15,,,,,,,,
072893,1,0.9120,10.77,8.79,8.69,9.36,8.54,,,,94.00,0.148,23.07,3.41,0.351,33.66,11.81,,,,,,,,
072893,1,1.1655,16.66,3.68,10.29,9.80,11.42,,,,116.00,0.199,28.04,5.58,0.2696,37.39,10.08,,,,,,,,
072893,1,3.3308,11.3,4.88,8.6,7.50,24.98,,,,38.00,0.19,25.35,4.82,0.5927,37.94,22.49,,,,,,,,
072893,1,0.7401,6.79,8.19,6.07,6.96,5.15,,,,54.00,0.176,18.78,3.31,0.3039,32.37,9.84,,,,,,,,
072893,1,0.8588,12.52,11.57,11.43,11.98,10.29,,,,121.00,0.064,24.25,1.55,0.1832,32.74,6.00,,,,,,,,
072893,1,0.8433,22.19,10.24,7.95,12.88,10.86,,,,95.00,0.145,18.88,2.74,0.1465,36.28,5.32,,,,,,,,
091693,0,2.3210,13.01,19,15.85,15.80,36.67,259,282,46,238.00,0.234,14.2,3.32,0.434,14.1,6.12,,,,,,,,
091693,0,5.0900,12.86,20.6,15.54,16.30,82.97,120,193,129,146.00,0.43,15,6.45,0.673,20,13.46,,,,,,,,
091693,0,1.0250,18.92,16.03,14.75,16.90,17.32,370,221,0,250.00,0.283,25.4,7.19,0.38,10.8,4.10,,,,,,,,
091693,0,3.2670,11.95,12.89,18.85,14.60,47.70,147,118,50,104.00,0.139,12.8,1.78,0.418,16.6,6.94,,,,,,,,
091693,0,2.4010,10.24,11.06,12.83,10.90,26.17,143,74,49,100.00,0.323,11.8,3.81,0.487,11.8,5.75,,,,,,,,
091693,0,2.4100,20.83,15.09,13.76,17.60,42.42,271,159,10,193.00,0.27,18.9,5.10,0.384,14.5,5.57,,,,,,,,
091693,0,1.1820,12.66,9.13,9.26,11.40,13.47,97,127,233,139.00,0.228,14.2,3.24,0.312,18.3,5.71,,,,,,,,
091693,0,2.6100,20.73,16,18.17,18.30,47.76,263,174,112,181.00,0.258,17.4,4.49,0.557,15.4,8.58,,,,,,,,
091693,1,1.8420,24.44,17.31,12.37,18.50,34.08,596,186,199,331.00,0.247,21.6,5.34,0.345,14.5,5.00,,,,,,,,
091693,1,1.0350,22.34,17.15,13.51,20.60,21.32,398,255,83,308.00,,,,0.24,13.6,3.26,,,,,,,,
091693,1,1.1050,37.89,28.26,25.09,30.10,33.26,910,855,322,737.00,0.206,25.1,5.17,0.276,16.8,4.64,,,,,,,,
091693,1,1.8140,22.81,15.3,15.42,17.10,31.02,353,142,333,249.00,0.201,15.9,3.20,0.496,19.6,9.72,,,,,,,,
091693,1,1.2400,19.44,16.38,13.22,17.40,21.58,284,227,31,224.00,0.406,20.9,8.49,0.245,15.1,3.70,,,,,,,,
091693,1,1.0610,29.03,26.1,15.18,25.30,26.84,340,193,0,228.00,0.145,17.5,2.54,0.251,20.8,5.22,,,,,,,,
091693,1,2.4280,24.26,21.22,8.8,20.90,50.75,351,279,0,273.00,0.183,11.6,2.12,0.332,17.4,5.78,,,,,,,,
052594,1,2.1617,27.08,23.83,14.14,22.16,47.90,355.2,279.57,164.32,272.47,,,,0.1173,33.97,3.98,,90.72,,,,,,
052594,1,1.0482,21.36,16.63,17.69,18.57,19.46,221.78,170.47,0,196.59,,,,0.0569,38.64,2.20,,184.03,,,,,,
052594,1,1.3621,35.43,25.53,26.16,29.20,39.77,295.43,316.17,314.91,308.50,,,,0.0918,33.65,3.09,,110.22,,,,,,
052594,1,0.5753,21.72,19.29,14.64,18.77,10.80,236.05,161.95,111.04,173.57,,,,0.0809,35.18,2.85,,16.09,,,,,,
052594,1,0.6687,24.69,23.96,19.17,22.61,15.12,237.12,194.33,175.31,202.62,,,,0.0722,34.78,2.51,,,,,,,,
052594,0,1.1415,17.72,8.59,7.48,12.11,13.82,122.23,26.2,25.92,66.19,,,,0.1393,38.58,5.37,,,,,,,,
052594,0,0.9338,7.75,5.88,6.41,6.67,6.23,24.38,14.65,28.38,22.61,,,,0.1951,33.21,6.48,,,,,,,,
052594,0,0.7607,12.48,9.32,11.87,11.19,8.51,76.13,54.18,58.21,62.56,,,,0.0664,29.34,1.95,,,,,,,,
052594,0,1.1309,9.04,10.63,10.91,10.07,11.39,129.31,184.73,157.52,155.17,,,,0.0678,22.93,1.55,,31.17,,,,,,
052594,0,1.2937,12.5,9.75,9.28,10.59,13.70,202.44,91.6,48.96,118.26,,,,0.1142,34.44,3.93,,60.19,,,,,,
052594,0,1.8481,13.53,9.77,9.06,10.71,19.79,270.74,129.2,116.56,169.42,,,,0.2255,30.13,6.79,,32.65,,,,,,
052594,0,1.5237,6.18,5.57,8.75,6.68,10.18,30.45,34.83,46.7,36.72,,,,0.2753,26.73,7.36,,4.58,,,,,,
052594,0,2.1696,19.71,11.6,8.59,13.24,28.73,,,,,,,,0.2797,36.96,10.34,,22.27,,,,,,
062294,0,1.4653,8.73,5.02,6.01,6.52,9.55,81.67,48.06,26.81,51.04,,,,0.3258,33.1,10.78,,,,,,,,
062294,0,4.0120,9.97,7.21,7.66,8.31,33.36,89.71,37.01,47.37,58.69,,,,0.8219,34.77,28.58,,,,,,,,
062294,0,2.0377,8.56,4.49,5.51,6.24,12.71,55.02,42.28,13.93,38.34,,,,0.3637,30.95,11.26,,,,,,,,
062294,0,0.9094,7.18,6.38,7.83,7.13,6.49,23.31,11.46,14.46,16.44,,,,0.1506,33.03,4.97,,,,,,,,
062294,0,2.1642,4.94,4.98,4.82,4.91,10.63,16.93,26.62,22.94,22.12,,,,0.4815,29.47,14.19,,,,,,,,
070694,0,1.1066,4.22,3.74,5.77,4.49,4.97,13.09,16.17,9.74,13.20,0.3143,16.81,5.28,0.2663,21.5,5.73,,0.00,,,,,,
070694,0,1.3515,6.63,6.2,9.02,7.14,9.66,62.7,37.71,57.77,52.57,0.3275,17.18,5.63,0.1995,22.74,4.54,,8.10,,,,,,
070694,0,1.7641,5.98,4.88,2.36,4.56,8.05,17.86,17.81,12.65,16.35,0.7452,15.35,11.44,0.5302,28.73,15.23,,0.77,,,,,,
070694,0,0.9747,6.72,6.68,5.87,6.45,6.28,19.31,19.6,22.44,20.37,0.3059,16.74,5.12,0.3288,23.51,7.73,,1.27,,,,,,
070694,0,1.5684,5.2,11.46,14.05,9.79,15.36,50.32,106.87,110.01,86.26,0.279,14.72,4.11,0.4101,21.05,8.63,,32.24,,,,,,
070694,1,1.1287,18.79,14.75,9.64,14.36,16.21,225.84,94.43,145.86,154.33,0.2307,19.42,4.48,0.4263,29.12,12.41,,45.01,,,,,,
070694,1,0.7977,9.31,9.79,10.02,9.70,7.74,103.02,82.79,90.85,92.08,0.1846,19.34,3.57,0.1967,23.2,4.56,,10.26,,,,,,
070694,1,1.0974,8.41,10.52,8.66,9.13,10.02,117.36,75.01,37.97,85.39,0.4796,18.51,8.88,0.4663,26.43,12.32,,16.03,,,,,,
070694,1,1.6074,8.56,9.18,9.87,9.13,14.67,138.96,100.62,95.68,113.70,0.2988,16.03,4.79,0.5995,23.53,14.11,,19.69,,,,,,
072694,0,1.2672,4.12,5.42,8.1,5.69,7.21,37.3,49.99,80.29,53.74,0.198,12.52,2.48,0.1447,18.7,2.71,,,,,,,,
072694,0,1.5468,10.54,9.69,10.2,10.13,15.67,189.02,185.99,132.5,174.08,0.2311,11.88,2.75,0.3134,23.27,7.29,,,,,,,,
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072694,0,1.8586,3.63,3.93,4.71,4.03,7.50,23.56,28.33,31.28,27.34,0.199,13.57,2.70,0.4502,17.52,7.89,,,,,,,,
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